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Image Search Results
Journal: Nature communications
Article Title: Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics.
doi: 10.1038/s41467-024-53683-7
Figure Lengend Snippet: Fig. 4 | Detection range and limitation. a Study design for comparative proteomic analysis across various tissue volumes using in-gel (blue) and in-tip (red) digestions. Peptides from different diameters are analyzed using data-dependent acquisition mass spectrometry (DDA-MS; n = 3 technical replicates) and data-independent acquisition mass spectrometry (DIA-MS; n = 3 technical replicates). b Number of identified peptides and proteins for varied tissue volumes using in-gel and in-tip digestions as shown in (a), including in-gel DDA-MS (light blue), in-gel DIA-MS (blue), in-tip DDA-MS (orange), and in-tip DIA-MS (red). n = 3 technical replicates for each method and tissue volume. Data were presented as mean values ± standard deviation. c Asialoglycoprotein receptor 1 (ASGR1; yellow) and nuclear (blue) staining image of a mouse liver formalin-fixed paraffin-embedded (FFPE) slice before gel-making (n = 1). d ASGR1 and nuclear staining image of a mouse liver FFPE slice after gel-making (n = 1). e ASGR1 and nuclear staining image of a mouse liver FFPE slice after drying onto laser capture microdissection frame slide (n = 1). Scale bars are provided. f Comparison of proteins between single nucleus (n = 5 biolo- gical replicates; blue) and single mononuclear hepatocyte (n = 6 biological
Article Snippet: The tissue section was incubated for 30min in citrate antigen retrieval solution (Leagene, China) and subsequently in blocking buffer (Sangon, China) at room temperature for 1 h. The section was then incubated with
Techniques: Data-dependent acquisition, Mass Spectrometry, Data-independent acquisition, Standard Deviation, Staining, Laser Capture Microdissection, Comparison